tm4 sertoli cell line  (ATCC)

Journal: Toxics

Article Title: PFOA Damages Blood–Testis Barrier Integrity in Mice by Inhibited Glycolysis Caused H3K18 Lactylation Modification Impairment

doi: 10.3390/toxics14050399

Figure Lengend Snippet: PFOA altered the expression of glycolysis-related genes. Based on RNA-seq analysis, the total RNA expression profiles of testicular tissues in the control and 5 mg/kg/day PFOA-exposed groups were compared ( A ). KEGG pathway enrichment ( B ) and GO functional enrichment analyses ( C ) of differentially expressed genes revealed that the glycolysis pathway served as a key target of PFOA. After 24 h of PFOA treatment in TM4 cells, cell viability was decreased ( D ), accompanied by significant changes in the expression of glycolysis-related genes ( E ). ( n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001, * vs. control group).

Article Snippet: This experiment utilized a mouse testicular sertoli cell line (TM4) obtained from the ATCC cell bank in the United States.

Techniques: Expressing, RNA Sequencing, RNA Expression, Control, Functional Assay

Journal: Toxics

Article Title: PFOA Damages Blood–Testis Barrier Integrity in Mice by Inhibited Glycolysis Caused H3K18 Lactylation Modification Impairment

doi: 10.3390/toxics14050399

Figure Lengend Snippet: PFOA inhibited the expression of glycolysis and BTB-related molecules in TM4 cells and mouse testicular tissues. In PFOA-treated TM4 cells, the protein levels of key glycolytic molecules, including HK1 ( B ), GLUT1 ( C ) and LDHA ( D ), were downregulated ( A – D ), accompanied by reduced production of lactate ( E ) and ATP ( F ). Meanwhile, both the gene ( G ) and protein ( H ) expression of BTB-related factors were decreased in TM4 cells. Consistent with the cellular results, the mRNA ( I ) and protein ( J ) levels of glycolysis-related molecules were also suppressed in mouse testis, along with a decline in lactate concentration ( K ). ( n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001, * vs. control group).

Article Snippet: This experiment utilized a mouse testicular sertoli cell line (TM4) obtained from the ATCC cell bank in the United States.

Techniques: Expressing, Concentration Assay, Control

Journal: Toxics

Article Title: PFOA Damages Blood–Testis Barrier Integrity in Mice by Inhibited Glycolysis Caused H3K18 Lactylation Modification Impairment

doi: 10.3390/toxics14050399

Figure Lengend Snippet: Effects of PFOA on H3K18 lactylation and the transcriptional regulation of key genes involved in glycolysis. PFOA exposure reduced the modification levels of Pan-Kla and H3K18la in TM4 cells ( A ) and mouse testicular tissues ( B ). ChIP-qPCR results further demonstrated that PFOA altered the binding of H3K18la to glycolytic genes, thereby regulating their transcription ( C ). ( n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001, * vs. control group).

Article Snippet: This experiment utilized a mouse testicular sertoli cell line (TM4) obtained from the ATCC cell bank in the United States.

Techniques: Modification, ChIP-qPCR, Binding Assay, Control

Journal: Toxics

Article Title: PFOA Damages Blood–Testis Barrier Integrity in Mice by Inhibited Glycolysis Caused H3K18 Lactylation Modification Impairment

doi: 10.3390/toxics14050399

Figure Lengend Snippet: Protective effects of Nala against PFOA-induced reproductive damage. Gradient Nala treatment for 24 h significantly elevated the levels of Pan-Kla and H3K18la in TM4 cells ( A ). After co-treatment with 30 mM Nala and 400 μM PFOA for 24 h, the PFOA-mediated reductions in Pan-Kla and H3K18la were notably restored ( B ). The decreased expression of Hk1, Glut1 and Ldha was partially rescued ( C ), and the downregulated mRNA ( D ) and protein ( E ) levels of BTB-related molecules were also markedly recovered. ( n = 3, * p < 0.05; ** p < 0.01; *** p < 0.001; * vs. control group. # p < 0.05; ## p < 0.01; ### p < 0.001; # vs. PFOA group).

Article Snippet: This experiment utilized a mouse testicular sertoli cell line (TM4) obtained from the ATCC cell bank in the United States.

Techniques: Expressing, Control

Journal: Molecular Metabolism

Article Title: AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet

doi: 10.1016/j.molmet.2026.102350

Figure Lengend Snippet: AMPK inhibition-induced Angiogenin is accompanied by alteration of tRFs profile in vitro. A Western blot results of proteins related with AMPK-mTOR pathways in TM3, TM4, and NIH/3T3 cell lines using compound C (CC), rapamycin (Rapa), and MHY1485 (MHY) in TM3, TM4, and NIH/3T3 cell lines (left to right). The number represents the densiometric comparison of the expression of pAMPK and pmTOR proteins against AMPK and mTOR, respectively. B Angiogenin gene expression. Gene expression was quantified after 12 h of treatment. mRNA expression level was normalized to Actin . Data are represented as mean ± standard deviation. Statistical analysis was performed using One-way ANOVA with Tukey post-hoc test. Different letters are significantly different. C Northern blot results of tRF3-Pro and Val in mouse testis cell lines. Each sample RNAs were extracted from both TM3 and TM4 cells with CC treatment and overexpression of Angiogenin (Ang OE). Each intact tRNA was shown as a control. Band intensities of Northern blot are represented as bar graph after quantification using Image J software. Data are presented as mean ± standard deviation. One-way ANOVA was used for statistical analysis, and the different alphabets with Tukey post-hoc test. D Distribution of read counts based on small RNA type. E Distribution of reads assigned to GtRNAdb in tRF subtypes. The total bar height is proportional to the total read count for each group. Percentages indicate the relative proportion of each tRF subtype within the corresponding group, and n represents the actual read count number for each subtype. tRF5, reads derived from tRNA 5′ end among reads aligned to GtRNAdb; tRF3, reads derived from tRNA 3′ end among reads aligned to GtRNAdb; other, not included in the previous two groups. F Histogram showing the distribution of log 2 (Fold change) using reads aligned to GtRNAdb. Green, CC-treated versus non-treated; Yellow, ANG overexpression versus control. G Volcano plot depicting significantly changed tRFs in TM4 cell lines. Results from CC-treated versus non-treated are represented as circles, and results from ANG overexpression versus control are represented as triangles. n = 2 for each group.

Article Snippet: The mouse testicular TM3 cell line and TM4 Sertoli cell line were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Inhibition, In Vitro, Western Blot, Comparison, Expressing, Gene Expression, Standard Deviation, Northern Blot, Over Expression, Control, Software, Derivative Assay